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Interferon alpha-2 (IFNA2) Antibody [N27]

In Stock
Catalog Number Formulation Size Price
3440-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3440-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3440-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
Positive Control
Stimulated human peripheral blood mononuclear cells.
Immunogen
Purified recombinant human IFN-alpha2
Alternate Names
Interferon alpha-2, Interferon alpha-A, Alpha 2a interferon; IFN alpha; IFN-alpha-2; IFNA; IFNA2; IFNA2B; Interferon alpha 2a; Interferon alpha 2b; Interferon alpha-2; Interferon alpha-A; LeIF2; LeIFA

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
N27
Chromosome Location
9q22
Mol. Weight of Antigen
16-27kDa

Functions

  • Produced by macrophages, IFN-alpha have antiviral activities.

Key References

  • Aman MJ et al. Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes. Blood 1996, 87(11):4731-4736
  • Kontsek P et al. Mapping of two immunodominant structures on human interferon alpha 2c and their role in binding to cells. Mol Immunol 1991, 28:1289-1297
  • Pestka S et al. Interferons and their actions. Annu Rev Biochem 1987, 56:727-777
  • Kontsek P et al. Peptide-mapping of three neutralizing epitopes into predicted biologically active sites of human interferon-alpha 2. Immunol Lett 1993, 35(3):281-284
  • Capon DJ et al. Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides. Mol Cell Biol 1985, 5(4):768-779
  • Sen GC et al.The interferon system. A bird's eye view of its biochemistry. J Biol Chem 1992, 267(8):5017-5020
  • Kurane I et al. Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes. J Exp Med 1987, 166(4):999-1010
  • Lepe-Zuniga JL et al. Production of interferon-alpha induced by dsRNA in human peripheral blood mononuclear cell cultures: role of priming by dsRNA-induced interferons-gamma and -beta. J Interferon Res 1989, 9(4):445-456

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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