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HLA-E (Major histocompatibility complex, class I, E) Antibody [HLAE/9468]

In Stock
Catalog Number Formulation Size Price
3133-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3133-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3133-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

HLA-E Antibody (3H2679) is an IgG1 κ mouse monoclonal HLA-E antibody (also designated HLA major histocompatibility complex, class I, E antibody, human leukocyte antigen (HLA) E antibody, 45 kDa transmembrane class I human leukocyte antigen E antibody, chromosome 6p22 gene complex class 1E antibody, or natural killer cell (NK) expansion marker antibody) that detects the HLA-E protein of human origin by WB, IP, IF and IHC(P). HLA-E Antibody (3H2679) is available as the non-conjugated anti-HLA-E antibody. Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs). MHC class I molecules consist of two polypeptide chains, an a or heavy chain and a non-covalently associated protein, b-2-Microglobulin. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A is a MHC class I heavy chain molecule that plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and HLA-C are proteins encoded by closely related genes that also exist in the MHC class I. HLA-E belongs to the HLA class I heavy chain paralogs. HLA-E is a heterodimer consisting of a heavy chain and a light chain. The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Secreted
Gene Name
Immunogen
Recombinant fragment (around aa1-150) of human HLAE protein (exact sequence is proprietary)
Alternate Names
major histocompatibility complex, class I, E, MHC class I antigen E; MHC class Ib antigen; non-classical MHC class I antigen; nonclassical MHC class I antigen, QA1; HLA-6.2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
HLAE/9468
Chromosome Location
6p22.1
Mol. Weight of Antigen
40kDa

Key References

  • Morinaga T, Iwatsuki M, Yamashita K, Matsumoto C, Harada K, Kurashige J, Iwagami S, Baba Y, Yoshida N, Komohara Y, Baba H. Evaluation of HLA-E Expression Combined with Natural Killer Cell Status as a Prognostic Factor for Advanced Gastric Cancer. Ann Surg Oncol. 2022 Aug;29(8):4951-4960. doi: 10.1245/s10434-022-11665-3. Epub 2022 Apr 12. PMID: 35412205.
  • Morinaga T, Iwatsuki M, Yamashita K, Yasuda-Yoshihara N, Yamane T, Matsumoto C, Harada K, Eto K, Kurashige J, Ishimoto T, Baba Y, Miyamoto Y, Yoshida N, Baba H. Dynamic Alteration in HLA-E Expression and Soluble HLA-E via Interaction with Natural Killer Cells in Gastric Cancer. Ann Surg Oncol. 2023 Feb;30(2):1240-1252. doi: 10.1245/s10434-022-12505-0. Epub 2022 Sep 23. PMID: 36149610.
  • He W, Gea-Mallorquí E, Colin-York H, Fritzsche M, Gillespie GM, Brackenridge S, Borrow P, McMichael AJ. Intracellular trafficking of HLA-E and its regulation. J Exp Med. 2023 Aug 7;220(8):e20221941. doi: 10.1084/jem.20221941. Epub 2023 May 4. PMID: 37140910; PMCID: PMC10165540.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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