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Heparan Sulfate Proteoglycan (Large) / Perlecan Antibody [SPM255]

In Stock
Catalog Number Formulation Size Price
3339-RTM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3339-RTM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3339-RTM1X-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Product Properties & Targets

Antibody Type
Host
Rat
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
Positive Control
Breast Carcinomas, squamous cell carcinomas.
Immunogen
Murine EHS laminin preparation
Alternate Names
Basement membrane-specific heparan sulfate proteoglycan core protein, Perlecan, Heparan sulfate proteoglycan of basement membrane; HSPG; Hspg2; LG3 peptide; Perlecan; PLC; SJS1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SPM255
Chromosome Location
1p36.1-p34
Mol. Weight of Antigen
>400kDa

Functions

  • Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
  • Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
  • The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.

Key References

  • Ljubimov et. al., Lab Invest, 1995; 72:461-473.
  • Couchman et. al., Matrix, 1989; 9:311-321.
  • Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113.
  • Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970.
  • Ljubimov et. al., Int J Cancer, 1992; 50:562-566.
  • Guelstein et. al., Int J Cancer, 1993; 53:269-277.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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