HCG-beta (Pregnancy & Choriocarcinoma Marker) [HCGb/1996R]

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Summary

This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Product Properties & Targets

Host
Species Reactivity
Isotype
IgG
Cellular Localization
NA
Gene Name
CGB3, CGB5, CGB8, NA
Mol. Weight of Antigen
22kDa
Immunogen
Recombinant full-length hCG beta protein

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
HCGb/1996R
Human Gene Symbol
CGB
Chromosome Location
19q13.33

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml
  • 30 min RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Cocquebert M et. al. Am J Physiol Endocrinol Metab. 2012;303(8):E950-8.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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