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Recombinant Glutathione S-Transferase Pi1 (GSTP1) Antibody [GSTP1/12956R]

In Stock
Catalog Number Formulation Size Price
2950-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2950-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2950-RBM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
GSTP1 Antibody in Immunohistochemistry (IHC (P))

GSTP1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Glutathione S-transferase pi 1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R). Inset: PBS instead of primary antibody; secondary only negative control.

GSTP1 Antibody in SDS-PAGE

GSTP1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Glutathione S-transferase pi 1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R). Confirmation of Purity and Integrity of Antibody.

GSTP1 Antibody in Western Blot (WB)

GSTP1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Rabbit Brain, Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart, Rabbit Heart, Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung and Rabbit Lung tissue lysates using GSTP1 Recombinant Rabbit Monoclonal Antibody (GSTP1/12956R).

GSTP1 Antibody Validation in Western Blot on Knockdown(KD) cells.

GSTP1 Antibody Validation in Western Blot on Knockdown(KD) cells.

Western Blot validation of anti-GSTP1 antibody. GSTP1 protein expression was compared in wild-type (WT) and GSTP1 shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. In response to oxidative stress, upregulation of the GST family member GSTP1 occurs, consistent with this function. Furthermore, the GSTP1 gene is subject to CpG island hyper-methylation, a state that correlates with human prostatic carcinogenesis. GSTP1 gene hyper-methylation can be detected in urine, ejaculate and plasma from men with prostate cancer, potentially making GSTP1 a useful biomarker for prostate cancer screening.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Knockdown
Species Reactivity
Human
Research Areas
Cancer, Metabolism
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Nucleus
Gene Name
GSTP1
Positive Control
Human brain, lung, placenta or liver.
Immunogen
Recombinant fragment (around aa19-206) of human full-length GSTP1 protein (exact sequence is proprietary)
Alternate Names
Glutathione S-transferase P, GST class-pi, GSTP1-1, EC:2.5.1.18, FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

Database Links

Entrez Gene ID
2950
SwissProt
P09211

Additional Information

Clone
GSTP1/12956R
Chromosome Location
11q13.2
Mol. Weight of Antigen
23kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All GSTP1 Antibodies

Functions

  • Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

Key References

  • Board, P.G., et al. 1992. The human Pi class glutathione transferasesequence at 12q13-q14 is a reverse-transcribed pseudogene. Genomics14: 470-473.
  • Klinga-Levan, K., et al. 1993. Mapping of glutathione transferase (GST)genes in the rat. Hereditas 119: 285-296.
  • Xia, C., et al. 1993. The human glutathione S-transferase P1-1 gene:modulation of expression by retinoic acid and Insulin. Biochem. J. 292:845-850.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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