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Glucose 6-Phosphate Isomerase Antibody [GPI/2439]

In Stock
Catalog Number Formulation Size Price
2821-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2821-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2821-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
GPI Antibody in Western Blot (WB)

GPI Antibody in Western Blot (WB)

Western blot analysis of Human kidney, Human Liver and Ramos tissue lysates using Glucose 6-Phosphate Isomerase Mouse Monoclonal Antibody (GPI/2439).

GPI Antibody in Immunohistochemistry (IHC (P))

GPI Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Glucose 6-Phosphate Isomerase Mouse Monoclonal Antibody (GPI/2439). Inset: PBS instead of primary antibody; secondary only negative control.

GPI in SDS-PAGE

GPI in SDS-PAGE

SDS-PAGE Analysis of Purified GPI Mouse Monoclonal Antibody (GPI/2439). Confirmation of Purity and Integrity of Antibody.

GPI Antibody in Western Blot (WB)

GPI Antibody in Western Blot (WB)

Western blot analysis of liver and kidney tissue lysates of different species using Glucose 6-Phosphate Isomerase Mouse Monoclonal Antibody (GPI/2439).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Besides it’s role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Colon Cancer, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Secreted
Gene Name
GPI
Positive Control
PC3, T47D, HepG2, MCF-7 or A549 cells. Human kidney, brain, pancreas or thyroid carcinoma. Liver, Ramos.
Immunogen
Recombinant human full-length GPI protein
Alternate Names
Glucose-6-phosphate isomerase, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF; aurocrine motility factor; EC 5.2.1.9; GNPI; Gpi1; hexose monophosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphosaccharomutase; SA-36; sperm antigen 36

Database Links

Entrez Gene ID
2821
SwissProt
P06744

Additional Information

Clone
GPI/2439
Chromosome Location
19q13.11
Mol. Weight of Antigen
55kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All GPI Antibodies

Functions

  • In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:3352745, PubMed:11004567). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:3352745, PubMed:11004567).

Key References

  • Beutler, E., et al. 1997.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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