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Recombinant FLI1 (Ewing s Sarcoma & Endothelial Marker) Antibody [FLI1/8318R]

In Stock
Catalog Number Formulation Size Price
2313-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2313-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2313-RBM13-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
Positive Control
Ewing s sarcoma (EWS) or angiosarcoma.|THP1 or Raji cells. Human tonsil
Immunogen
Recombinant fragment (around aa200-400) of human FLI1 protein (exact sequence is proprietary)
Alternate Names
Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, ERGB transcription factor; Ewing Sarcoma breakpoint region 2 (EWSR2); FLI1; FLI1 EWS fusion gene; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC1; Transcription factor ERGB; Viral integration region FLI1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
FLI1/8318R
Chromosome Location
11q24.1-q24.3
Mol. Weight of Antigen
51kDa

Functions

  • Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Key References

  • SabrinaRossi, et al. Modern Pathology(2004)17,547-552.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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