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Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody [rFLG/1945]

In Stock
Catalog Number Formulation Size Price
2312-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2312-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2312-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Product Properties & Targets

Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasmic granule
Gene Name
FLG
Positive Control
U-251-MGor A-431 cells. Human skin.
Immunogen
Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary)
Alternate Names
Filaggrin, ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rFLG/1945
Chromosome Location
1q21.3
Mol. Weight of Antigen
26-45kDa (Processed); 350kDa (Profilaggrin)

Functions

  • Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Key References

  • Gan, S.Q., et al. 1990. Biochemistry 29: 9432-9440.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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