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Recombinant ERG (Vascular Endothelial & Prostate Marker) Antibody [rERG/6843]

In Stock
Catalog Number Formulation Size Price
2078-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2078-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2078-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

ERG (ETS-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including hemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma, gastrointestinal stromal tumor, synovial sarcoma, meningioma, epithelioid sarcoma, malignant rhabdoid tumor, acute myeloid leukemia and blastic extramedullary myeloid tumor, and rarely Ewing sarcoma / primitive peripheral neuroectodermal tumor, chondrosarcoma, osteosarcoma, and rhabdomyosarcoma. For the identification of endothelial differentiation ERG seems more sensitive and specific than any other marker. Moreover, the interpretation is often easier due to the nuclear reaction, which also allows for double stains with cytoplasmic markers like podoplanin. Among carcinomas, ERG is highly specific for prostate, while the sensitivity is moderate.

Product Properties & Targets

Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
ERG
Positive Control
Ewing sarcoma or prostate adenocarcinoma tissues., Human tonsil
Immunogen
Recombinant fragment (around aa450 to 479) (C-terminus) of human ERG (exact sequence is proprietary)
Alternate Names
Transcriptional regulator ERG, Transforming protein ERG, ERG; ERG/EWS fusion gene; ERG/FUS fusion gene; ERG/TMPSSR2 fusion gene; ERG1; ETS-related; KCNH2; Oncogene ERG; p55; TMPRSS2/ERG fusion

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rERG/6843
Chromosome Location
21q22.2
Mol. Weight of Antigen
55kDa

Functions

  • Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

Key References

  • Miettinen M. Histopathology. 2014 Jan;64(1):101-18.
  • Stockman DL, Mod Pathol. 2014 Apr;27(4):496-501.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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