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Recombinant Eosinophil Peroxidase (EPX) Antibody [rEPO104]

In Stock
Catalog Number Formulation Size Price
8288-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8288-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8288-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Product Properties & Targets

Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasmic granule
Gene Name
EPX
Positive Control
MCF-7 cells. Tonsil
Immunogen
Human eosinophils from a patient with hypereosinophilic syndrome
Alternate Names
Eosinophil peroxidase, Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rEPO104
Chromosome Location
17q22
Mol. Weight of Antigen
70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)

Functions

  • Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.

Key References

  • Skubitz, K.M., et al. 1989. J. Leukocyte Biol. 46:109-118.
  • Skubitz, K.M., et al. 1988. J. leukocyte Bio. 44:158-165.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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