EME1 [MTA31 7H2/1]



Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Product Properties & Targets

Species Reactivity
Cellular Localization
Nucleolus, Nucleus
Light Chain
Gene Name
Positive Control
HEK293T or U-87 MG cells. Human tonsil.
His-tagged recombinant Eme1 of human origin
Alternate Names
Crossover junction endonuclease EME1, MMS4 homolog, Crossover junction endonuclease EME1; Essential Meiotic Endonuclease 1 Homolog 1/2; hMMS4; MMS4L; SLX2A

Database Links

Entrez Gene ID

Additional Information

MTA31 7H2/1
Human Gene Symbol
Chromosome Location
Mol. Weight of Antigen


  • Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Known Applications & Suggested Dilutions

  • Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
  • ,Western Blot (1-2ug/ml)
  • Immunofluorescence (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Tomoda, Y., et al. 2009. Functional evidence for Eme1 as a marker of cisplatin resistance. Int. J. Cancer 124: 2997-3001.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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