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EME1 Antibody [MTA31 7H2/1]

In Stock
Catalog Number Formulation Size Price
146956-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
146956-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
146956-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleolus, Nucleus
Gene Name
Positive Control
HEK293T or U-87 MG cells. Human tonsil.
Immunogen
His-tagged recombinant Eme1 of human origin
Alternate Names
Crossover junction endonuclease EME1, MMS4 homolog, Crossover junction endonuclease EME1; Essential Meiotic Endonuclease 1 Homolog 1/2; hMMS4; MMS4L; SLX2A

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MTA31 7H2/1
Chromosome Location
17q21.33
Mol. Weight of Antigen
65kDa

Functions

  • Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Key References

  • Tomoda, Y., et al. 2009. Functional evidence for Eme1 as a marker of cisplatin resistance. Int. J. Cancer 124: 2997-3001.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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