SDS-PAGE Analysis Purified DAZL Mouse Monoclonal Antibody (DAZL/4253) Confirmation of Integrity and Purity of Antibody.

Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).

Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).

Printable Datasheet

DAZL (Deleted in Azoospermia-like) [DAZL/4253]

Name: DAZL (Deleted in Azoospermia-like)
SKU: 1618-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Product Properties & Targets

Host

Mouse

Species Reactivity

Cynomolgus Monkey, Human, Mouse, Rat

Isotype

IgG1

Cellular Localization

Cytoplasm, Nucleus

Gene Name

DAZL

Positive Control

Human testis or ovary tissue.

Immunogen

Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV.

Alternate Names

Deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, Deleted in azoospermia-like, DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZH, DAZL1, DAZLA, SPGYLA

Database Links

Entrez Gene ID

1618

Unigene

131179

SwissProt

Q92904

Additional Information

Clone

DAZL/4253

Human Gene Symbol

DAZL

Chromosome Location

3p24.3

Mol. Weight of Antigen

33kDa

Functions

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts (By similarity).

Known Applications & Suggested Dilutions

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Anderson, R.A., et al. (2007). Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis. BMC Developmental Biology, 7, 136.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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