Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CK LMW Rabbit Recombinant Monoclonal Antibody (KRTL/1577R).

SDS-PAGE Analysis Purified CK LMW Rabbit Recombinant Monoclonal Antibody (KRTL/1577R). Confirmation of Purity and Integrity of Antibody.

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) [KRTL/1577R]

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Summary

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Dog, Human
Research Areas
Autophagy
Isotype
IgG
Cellular Localization
cornified envelope, Cytoskeleton, Cytosol, Extracellular exosome, keratin filament
Gene Name
KRT77
Mol. Weight of Antigen
56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
Immunogen
Recombinant human KRT77 protein fragment

Database Links

Entrez Gene ID
374454
Unigene
334989
SwissProt
Q7Z794

Additional Information

Clone
KRTL/1577R
Human Gene Symbol
KRT77
Chromosome Location
17q21.2

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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