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Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [rKRT7/8763]

In Stock
Catalog Number Formulation Size Price
3855-MSM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3855-MSM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3855-MSM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CK7 Recombinant Mouse Monoclonal Antibody (rKRT7/8763). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CK7 Recombinant Mouse Monoclonal Antibody (rKRT7/8763). Confirmation of Purity and Integrity of Antibody.

Cytokeratin 7 Antibody in Western Blot (WB)

Cytokeratin 7 Antibody in Western Blot (WB)

Western Blot Analysis of human lung tissue ysate using Cytokeratin-7 Recombinant Mouse Monoclonal Antibody (rKRT7/8763).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract.This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm.
Gene Name
KRT7
Positive Control
Carcinoma of ovary|Cervix|lung|or breast, Lung.
Immunogen
Recombinant full-length human KRT7 protein
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Database Links

Entrez Gene ID
3855
SwissProt
P08729Human

Additional Information

Clone
rKRT7/8763
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa

Related Antibodies

View All KRT7 Antibodies

Functions

  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Key References

  • Ramaekers F., et al. Am J Pathol 1990;136:641-55

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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