
These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE
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Flow Cytometric Analysis of PFA-fixed HeLa cells. CEBPZ Mouse Monoclonal Antibody (PCRP-CEBPZ-2D8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

SDS-PAGE Analysis of Purified CEBPZ Mouse Monoclonal Antibody (PCRP-CEBPZ-2D8). Confirmation of Purity and Integrity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using CEBPZ Mouse Monoclonal (PCRP-CEBPZ-2D8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
C/EBP ζ (CCAAT/enhancer-binding protein ζ), also known as CBF, CBF2, NOC1 or HSP-CBF is a 1,054 amino acid nuclear protein belonging to the CBF/MAK21 family. C/EBP ζ stimulates transcription from the HSP70 and HSP40 promoters in a NF-Y dependent manner, requiring an intact NF-Y trimer which binds to the DNA. C/EBP ζ is thought to be a potential tumor suppressor gene, and aberrant methylation of the C/EBP ζ promoter may be involved with acute myeloid leukemia. The C/EBP ζ gene is conserved in a wide number of species including chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish and C. elegans and is located on human chromosome 2. Chromosome 2, the second largest human chromosome, consists of 237 million bases encoding over 1,400 genes, comprising approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2.
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