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Formalin-fixed, paraffin-embedded human tonsil stained with CD5L Mouse Monoclonal Antibody (CD5L/4420) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Stained using CD5L/4420 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified CD5L Mouse Monoclonal Antibody (CD5L/4420). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to CD5L (CD5L/4420). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
CD5L (CD5 molecule-like), also known as API6, PRO229, Sp伪 or SP-ALPHA, is a 347 amino acid secreted protein that belongs to the scavenger receptor cysteine-rich (SRCR) family of leukocyte regulating proteins. Expressed in bone marrow, spleen, thymus, lymph node and fetal liver, CD5L is thought to be involved in regulating the immune system via binding to peripheral monocytes and mediating their activation and overall survival. CD5L has three cysteine-rich domains and, in addition to its role in the immune system, may function to inhibit apoptosis and promote macrophage survival.
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