Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [MSVA-056R]

In Stock
Catalog Number Formulation Size Price
4684-RBM50-P0
Purified Ab with BSA and Azide
20ug
$324.50
4684-RBM50-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
NCAM1 Antibody in Numerous Tissues (IHC)

NCAM1 Antibody in Numerous Tissues (IHC)

Neural cell adhesion molecule 1 Rabbit Recombinant Monoclonal Antibody (MSVA-056R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Immunology, Neuroscience, Cytokine Signaling, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation, Neural Stem Cells, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
NCAM1
Positive Control
Tonsil: A small subset of (mainly interfollicular) lymphocytes should show a moderate to strong CD56 immunostaining. A weak to moderate staining can also be seen in fibroblastic reticular cells.
Immunogen
Recombinant fragment (around aa200-400) of human NCAM1 (CD56) protein (exact sequence is proprietary)
Alternate Names
Neural cell adhesion molecule 1, NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

Database Links

Entrez Gene ID
4684
SwissProt
P13591 & P13592

Additional Information

Clone
MSVA-056R
Chromosome Location
11q23.1
Mol. Weight of Antigen
145 and 125kDa|180

Related Antibodies

View All NCAM1 Antibodies

Functions

  • This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • (Microbial infection) Acts as a receptor for rabies virus.
  • (Microbial infection) Acts as a receptor for Zika virus.

Key References

  • Hilkens et al., Int J Cancer. 1984 34:197-206.
  • Kibbelaar et al. Eur. J. Cancer 1991 27:431-435.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales