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Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [MSVA-056R]

Additional information

Catalog Number: 4684-RBM50
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4684-RBM50-P0
Purified Ab with BSA and Azide
20ug
$324.50
4684-RBM50-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
NCAM1 Antibody in Numerous Tissues (IHC)

NCAM1 Antibody in Numerous Tissues (IHC)

Neural cell adhesion molecule 1 Rabbit Recombinant Monoclonal Antibody (MSVA-056R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-056R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
NCAM1
Positive Control
Tonsil: A small subset of (mainly interfollicular) lymphocytes should show a moderate to strong CD56 immunostaining. A weak to moderate staining can also be seen in fibroblastic reticular cells.
Immunogen
Recombinant fragment (around aa200-400) of human NCAM1 (CD56) protein (exact sequence is proprietary)
Expression System
CHO
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • (Microbial infection) Acts as a receptor for rabies virus.
  • (Microbial infection) Acts as a receptor for Zika virus.
Key References
  • Hilkens et al., Int J Cancer. 1984 34:197-206.
  • Kibbelaar et al. Eur. J. Cancer 1991 27:431-435.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4684
SwissProt
P13591 & P13592
Chromosome Location
11q23.1
Mol. Weight of Antigen
145 and 125kDa|180
Alternate Names
Neural cell adhesion molecule 1, NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

Summary

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

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