SDS-PAGE Analysis Purified CD35 Recombinant Rabbit Monoclonal Antibody (CD35/7016R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human kidney stained with CD35 Recombinant Rabbit Monoclonal Antibody (CD35/7016R) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney stained with CD35 Recombinant Rabbit Monoclonal Antibody (CD35/7016R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Printable Datasheet

Recombinant CD35 / CR1 (Follicular Dendritic Cell Marker) [CR1/7016R]

Name: CD35 / CR1 (Follicular Dendritic Cell Marker)
SKU: 1378-RBM17
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

CD35, complement receptor 1, is a cell membrane-bound, monomeric glycoprotein on numerous cell types including erythrocytes, leukocytes, glomerular podocytes, and follicular dendritic cells. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules.CD35 antigen is found on erythrocytes, B cells, a subset of T cells, monocytes, as well as eosinophils, and neutrophils. Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Membrane

Light Chain

kappa

Gene Name

CR1

Positive Control

Follicular dendritic cells (FDC) in tonsil.

Immunogen

Recombinant full-length human CR1 protein

Alternate Names

Complement receptor type 1, C3b/C4b receptor, C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen

Database Links

Entrez Gene ID

1378

Unigene

334019

SwissProt

P17927

Additional Information

Clone

CR1/7016R

Human Gene Symbol

CR1

Chromosome Location

1q32.2

Mol. Weight of Antigen

224kDa

Functions

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728).
(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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