Formalin-fixed, paraffin-embedded human tonsil stained with BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R).

SDS-PAGE Analysis Purified BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of PFA-fixed K562 cells. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-Rabbit IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed K562 cells labeling BCL10. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-rabbit IgG-CF488 (green). Phalloidin counterstain (red).

BCL10 (MALT-Lymphoma Marker) [BL10/2988R]

Name: BCL10 (MALT-Lymphoma Marker)
SKU: 8915-RBM3
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NF B activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytoplasm, Membrane raft, Perinuclear region

Gene Name

BCL10

Mol. Weight of Antigen

33kDa

Immunogen

Recombinant human BCL10 protein fragment (around aa122-168) (exact sequence is proprietary)

Database Links

Entrez Gene ID

8915

Unigene

193516

SwissProt

O95999

Additional Information

Clone

BL10/2988R

Human Gene Symbol

BCL10

Chromosome Location

1p22.3

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Ye H et. al. Am J Pathol 2000;157:1147-54.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.