Formalin-fixed, paraffin-embeddedhuman spleen stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).

SDS-PAGE Analysis Purified Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human MCF-7 cell lysate using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).

Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).

Printable Datasheet

Bcl-2 (Apoptosis & Follicular Lymphoma Marker) [BCL2/1878R]

Name: Bcl-2 (Apoptosis &amp; Follicular Lymphoma Marker)
SKU: 596-RBM8
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane

Gene Name

BCL2

Mol. Weight of Antigen

25-26kDa

Immunogen

Recombinant full-length human bcl-2 protein

Database Links

Entrez Gene ID

596

Unigene

150749

SwissProt

P10415

Additional Information

Clone

BCL2/1878R

Human Gene Symbol

BCL2

Chromosome Location

18q21.33

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Adams, J.M., et al. 1998. The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322-1326.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.