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Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody [100/D5]

In Stock
Catalog Number Formulation Size Price
596-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
596-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
596-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody

Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 Mouse Monoclonal Antibody (100/D5).

Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody - Image 2

Intracellular staining of Bcl-2 in Jurkat cells. Bcl-2 Mouse Monoclonal Antibody (100/D5) (red); isotype control (green).

Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified Bcl-2 Mouse Monoclonal Antibody (100/D5). Confirmation of Integrity and Purity of Antibody.

Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody - Image 4

Western blot analysis of MCF-7 cell lysate using Bcl-2 Mouse Monoclonal Antibody (100/D5).

BCL2 Antibody in SDS-PAGE

BCL2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Apoptosis regulator Bcl-2 Mouse Monoclonal Antibody (100/D5). Confirmation of Purity and Integrity of Antibody.

bcl-2 Antibody in Western Blot (WB)

bcl-2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Skeletal muscle, Human Spleen and Jurkat lysates using bcl-2 Mouse Monoclonal Antibody (100/D5).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Immunology, AKT Signaling, Basal Cell Marker, Cytokine Signaling, Lung Cancer, Mitochondria Marker, Neuroinflammation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane
Gene Name
BCL2
Positive Control
Jurkat or HeLa cells. Human tonsil or follicular lymphomas., MCF-7, Skeletal muscle, Spleen.
Immunogen
A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Alternate Names
Apoptosis regulator Bcl-2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Database Links

Entrez Gene ID
596
SwissProt
P10415

Additional Information

Clone
100/D5
Chromosome Location
18q21.33
Mol. Weight of Antigen
25-26kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All BCL2 Antibodies

Functions

  • Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Key References

  • Pezzella F et. al. American Journal of Pathology, 1990, 137(2):225-32.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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