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ATM / ATM serine / threonine kinase Antibody [ATM/9472]

In Stock
Catalog Number Formulation Size Price
472-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
472-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
472-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
ATM Antibody in SDS-PAGE

ATM Antibody in SDS-PAGE

SDS-PAGE Analysis Purified ATM Mouse Monoclonal Antibody (ATM/9472). Confirmation of Purity and Integrity of Antibody.

ATM Antibody in Immunohistochemistry (IHC (P))

ATM Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human adrenal gland stained with ATM Mouse Monoclonal Antibody (ATM/9472). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Epigenetics
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Cytoplasmic vesicle, Nucleus
Gene Name
ATM
Immunogen
Recombinant fragment (around aa1900-2500) of human ATM protein (exact sequence is proprietary)
Alternate Names
serine-protein kinase ATM, A-T mutated antibody; A-T mutated homolog antibody; AT mutated antibody; AT1 antibody; ATA antibody; Ataxia telangiectasia mutated antibody; Ataxia telangiectasia mutated gene antibody; Ataxia telangiectasia mutated homolog (human) antibody; Ataxia telangiectasia mutated homolog antibody; ATC antibody ATD antibody; ATDC antibody; ATE antibody ATM antibody; ATM serine/threonine kinase antibody; ATM_HUMAN antibody; DKFZp781A0353 antibody; MGC74674 antibody; OTTHUMP00000232981 antibody; Serine protein kinase ATM antibody; Serine-protein kinase ATM antibody; Serine/threonine-protein kinase ATM antibody; Tefu antibody TEL1 antibody; TEL1, telomere maintenance 1, homolog antibody; TELO1 antibody Telomere fusion protein antibody, AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Database Links

Entrez Gene ID
472
SwissProt
Q13315

Additional Information

Clone
ATM/9472
Chromosome Location
11q22.3
Mol. Weight of Antigen
370kDa

Related Antibodies

View All ATM Antibodies

Functions

  • Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.

Key References

  • De Sarkar N, Dasgupta S, Chatterjee P, et al. Genomic attributes of homology-directed DNA repair deficiency in metastatic prostate cancer. JCI Insight. 2021;6(23):e152789. Published 2021 Dec 8. doi:10.1172/jci.insight.152789
  • De Sarkar N, Dasgupta S, Chatterjee P, et al. Genomic attributes of homology-directed DNA repair deficiency in metastatic prostate cancer. JCI Insight. 2021;6(23):e152789. Published 2021 Dec 8. doi:10.1172/jci.insight.152789
  • De Sarkar N, Dasgupta S, Chatterjee P, et al. Genomic attributes of homology-directed DNA repair deficiency in metastatic prostate cancer. JCI Insight. 2021;6(23):e152789. Published 2021 Dec 8. doi:10.1172/jci.insight.152789

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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