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Recombinant AIF1 / Iba1 (Microglia Marker) Antibody [AIF1/8970R]

In Stock
Catalog Number Formulation Size Price
199-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
199-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
199-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant AIF1 / Iba1 (Microglia Marker) Antibody

Formalin-fixed, paraffin-embedded human spleen stained with AIF1 / Iba1 Recombinant Rabbit Monoclonal Antibody (AIF1/8970R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant AIF1 / Iba1 (Microglia Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified AIF1 / Iba1 Recombinant Rabbit Monoclonal Antibody (AIF1/8970R). Confirmation of Purity and Integrity of Antibody.

AIF1 Antibody in Western Blot (WB)

AIF1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Liver, Human Kidney, Human Spleen and Raw lysates using AIF1 Rabbit Recombinant Monoclonal Antibody (AIF1/8970R).

AIF1 Antibody in Western Blot (WB)

AIF1 Antibody in Western Blot (WB)

Western Blot Analysis of Brain, Kidney and Spleen tissue lysates of different species using AIF1 Rabbit Recombinant Monoclonal Antibody (AIF1/8970R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Dendritic Cell Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm. Cell surface.
Gene Name
AIF1
Positive Control
Human lymph node|tonsil or kidney. Human Brain, Human Liver, Human Spleen and Raw
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Alternate Names
Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Database Links

Entrez Gene ID
199
SwissProt
P55008

Additional Information

Clone
AIF1/8970R
Chromosome Location
6p21.33
Mol. Weight of Antigen
17kDa

Related Antibodies

View All AIF1 Antibodies

Functions

  • Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Key References

  • Kelemen SE,Autieri MV.Expression of allograft inflammatory factor-1 in T lymphocytes.Am J Pathol2005;167:619-26.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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