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ABCC4 / ATP Binding Cassette Subfamily C Member 4 (Drug Target) Antibody [ABCC4/9178 ]

In Stock
Catalog Number Formulation Size Price
10257-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10257-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10257-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter), and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues, yet at much lower levels of expression.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
Human prostate.
Immunogen
Recombinant fragment (around aa1-200) of human ABCC4 protein (exact sequence is proprietary)
Alternate Names
ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B, Multidrug resistance-associated protein 4, ABCC4; bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4); Canalicular multispecific organic anion transporter ABC superfamily; EST170205; MOATB; MRP/cMOAT related ABC transporter; Multi-specific organic anion transporter B; Multidrug resistance associated protein 4

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
ABCC4/9178
Chromosome Location
13q32.1
Mol. Weight of Antigen
150kDa

Functions

  • ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12883481, PubMed:12523936, PubMed:12835412, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12883481, PubMed:12523936, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:18300232, PubMed:17344354). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685).

Key References

  • Versantvoort, C.H., et al. 1995. Regulation by glutathione of drug transport in multidrug-resistant human lung tumour cell lines overexpressing multidrug resistance-associated protein. Br. J. Cancer 72: 82-89.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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