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ABCC4 / ATP-binding cassette sub-family C member 4 Antibody [ABCC4/9017]

Additional information

Catalog Number: 10257-MSM1
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
10257-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10257-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10257-MSM1-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
ABCC4 Antibody in Immunohistochemistry (IHC (P))

ABCC4 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human prostate stained with ABCC4 Mouse Monoclonal Antibody (ABCC4/9017). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

ABCC4 Antibody in SDS-PAGE

ABCC4 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified ABCC4 Mouse Monoclonal Antibody (ABCC4/9017). Confirmation of Purity and Integrity of Antibody.

ABCC4 Antibody in Western Blot (WB)

ABCC4 Antibody in Western Blot (WB)

Western blot analysis of kidney tissue lysates from human, rat, hamster and rabbit using ABCC4 Mouse Monoclonal Antibody (ABCC4/9017).

ABCC4 Antibody in Western Blot (WB)

ABCC4 Antibody in Western Blot (WB)

Western blot analysis of human kidney tissue lysates and HEK293 cell lysates using ABCC4 Mouse Monoclonal Antibody (ABCC4/9017).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ABCC4/9017
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Basolateral cell membrane
Gene Name
ABCC4
Positive Control
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
Immunogen
Recombinant fragment (around aa 1-110) of human ABCC4 protein
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12523936, PubMed:12835412, PubMed:12883481, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12523936, PubMed:12883481, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:17344354, PubMed:18300232). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10257
SwissProt
O15439
Chromosome Location
13q32.1
Mol. Weight of Antigen
149.52kDa
Alternate Names
ATP-binding cassette sub-family C member 4, MOAT-B|MOATB|MRP4

Summary

ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12523936, PubMed:12835412, PubMed:12883481, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12523936, PubMed:12883481, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:17344354, PubMed:18300232). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685).

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