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Home >> Antibodies >> von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rVWF/1465]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465).

img

Western Blot Analysis A) Recombinant Protein (B) human lung lysate Using Recombinant Mouse Monoclonal Antibody (rVWF/1465).

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SDS-PAGE Analysis Purified vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Positive Control

HUVEC cells. Tonsil.

Known Applications & Suggested Dilutions

Western Blot, IP, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunoprecipitation (0.5-1ug/500ug protein lysate)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

250kDa

Specificity & Comments

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Related Products



200ug/ml of recombinant Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Motta, A. et al. 2009. J Biomater Sci Polym Ed. 20: 1875-1897.
  2. Germann, B. et al. 2008. Pharmazie. 63: 303-307.

Bioinformatics

  •  Entrez Gene ID: 7450
  •  Gene Symbol: VWF
  •  SwissProt: P04275
  •  Chromosome Location: 12p13.31
  •  Unigene: 440848
NBT01540