Home >> Antibodies >> Transgelin (SM22-alpha)

Transgelin (SM22-alpha)

Mouse Monoclonal Antibody [Clone TAGLN/247]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human kidney stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247).

Formalin-fixed, paraffin-embedded human kidney stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Transgelin Mouse Monoclonal Antibody (TAGLN/247).

Product Details

Synonyms

22kDa actin-binding protein; Human 22kDa smooth muscle protein; Protein WS3-10; SMCC; Smooth muscle protein 22-alpha (SM22-alpha); TAGLN; TAGLN1; transgelin; Transgelin variant 2; WS3-10

Positive Control

U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human transgelin (TAGLN) protein.

Cellular Localization

Cytoplasm.

Species Reactivity

Human. Cow. Pig. Rabbit. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

22kDa

Specificity & Comments

This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.