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Home >> Antibodies >> TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker)

TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker)

Recombinant Mouse Monoclonal Antibody [Clone rACP5/1070]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Mouse Recombinant Monoclonal Antibody (rACP5/1070).

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SDS-PAGE Analysis of Purified TRAcP Mouse Recombinant Monoclonal Antibody (rACP5/1070). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using TRAcP Mouse Recombinant Monoclonal Antibody (rACP5/1070).

Product Details

Synonyms

Acid phosphatase 5, tartrate resistant; ACP5; serum band 5 tartrate-resistant acid phosphatase; SPENCDI; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP5; TRAcP; TRAP; TrATPase; Type 5 acid phosphatase

Positive Control

HepG2, 293T, K562 or RPMI-8226 cells. Spleen from Hairy Cell Leukemia (HCL) patient.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ACP5 protein

Cellular Localization

Cytoplasmic (Lysosomes)

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher's Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Capeller, B., Caffier, H., Sutterlin, M.W. and Dietl, J. 2003. Evaluation of tartrate-resistant acid phosphatase (TRAP) 5b as serum marker of bone metastases in human breast cancer. Anticancer Res. 23: 1011-1015.

Bioinformatics

  •  Entrez Gene ID: 54
  •  Gene Symbol: ACP5
  •  SwissProt: P13686
  •  Chromosome Location: 19p13.2
  •  Unigene: 1211
NBT02337