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Home >> Antibodies >> Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker)

Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker)

Mouse Monoclonal Antibody [Clone P-GF.44C]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (P-GF.44C).

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SDS-PAGE Analysis Purified Thymidine Phosphorylase / PD-ECGF Mouse Monoclonal Antibody (P-GF.44C). Confirmation of Integrity and Purity of Antibody

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Western Blot Analysis of human spleen tissue lysate using Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (P-GF.44C).

Product Details

Synonyms

ECGF; ECGF1; Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PD-ECGF; PDECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; Tymp

Positive Control

HUVEC cells. Breast,Spleen, Bladder, Lung or Kaposi tumors.

Known Applications & Suggested Dilutions

Western Blot, IP, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunoprecipitation (0.5-1ug/500ug protein lysate)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Cellular Localization

Cytoplasmic and Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. O'Brien TS, et. al. Cancer Research, 1996, 56(20):4799-804.
  2. Fox SB, et. al. Journal of Pathology, 1995, 176:183-90.
  3. Fox SB, et. al. British Journal of Cancer, 1996, 73:275-80

Bioinformatics

  •  Entrez Gene ID: 1890
  •  Gene Symbol: TYMP
  •  SwissProt: P19971
  •  Chromosome Location: 22q13.33
  •  Unigene: 180903
NBT01244