2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> POU1F1 / PIT-1

POU1F1 / PIT-1

Mouse Monoclonal Antibody [Clone PIT1/7262]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human pituitary stained with POU1F1 / PIT-1 Mouse Monoclonal Antibody (PIT1/7262). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Dwarf; GHF1A; Growth hormone factor 1; Hmp1; PiT-1; Pit1 beta; PIT1Z; Pituitary growth hormone; Pituitary specific positive transcription factor 1; POU domain class 1 transcription factor 1; POU domain transcriptional regulator; POU1F1

Positive Control

Human pituitary or tonsil.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1:10-1:20 for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length POU1F1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

31-35kDa

Specificity & Comments

Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain, which consists of two highly conserved regions, tethered by a variable linker of 14-26 amino acids. Pit-1, also known as growth hormone factor-1 (GHF-1), a member of the POU homeodomain family, is essential for the normal development of the anterior pituitary gland, where it is required for the formation of somatotropes, lactotropes and thyrotropes. In somatotropes and lactotropes, Pit-1 activates the production of growth hormone and Prolactin, respectively. In addition, Pit-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated Pit-1a and Pit-1b, which differ in their trans-activation ability. Mutations in the Pit-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin and thyroid stimulating hormone. The gene which encodes Pit-1 maps to human chromosome 3p11.2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yao, J., et al. 2016. Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus. J. Clin. Invest. 126: 732-744.

Bioinformatics

  •  Entrez Gene ID: 5449
  •  Gene Symbol: POU1F1
  •  SwissProt: P28069
  •  Chromosome Location: 3p11.2
  •  Unigene: 591654