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Home >> Antibodies >> PHF10(Transcription Factor)

PHF10(Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-PHF10-2A10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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SDS-PAGE Analysis PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PHF10-Monospecific Mouse Monoclonal Antibody (PCRP-PHF10-2A10).

Product Details

Synonyms

BAF45a; BRG1-associated factor 45a; FLJ10975; MGC111009; PHD finger protein 10; PHD finger protein 10, isoform a; PHD zinc finger protein XAP135; Phf10; XAP135

Positive Control

HeLa cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length humanPHF10protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to react in Mouse and Chick.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

56.05kDa

Specificity & Comments

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 55274
  •  Gene Symbol: PHF10
  •  SwissProt: Q8WUB8
  •  Chromosome Location: 6q27
  •  Unigene: 435933