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Home >> Antibodies >> OCT-4 (POU5F1) (Germ Cell Tumor Marker)

OCT-4 (POU5F1) (Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone OCT4/3508]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human seminoma stained with OCT-4 Mouse Monoclonal Antibody (OCT4/3508) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human seminoma stained with OCT-4 Mouse Monoclonal Antibody (OCT4/3508) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

OCT3; OCT3A; OCT4; OCT4A; OCT-3/4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Positive Control

Raji or U-937 cells. Human seminoma or testicular carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

38.6kDa

Specificity & Comments

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.

Bioinformatics

  •  Entrez Gene ID: 5460
  •  Gene Symbol: POU5F1
  •  SwissProt: Q01860
  •  Chromosome Location: 6p21.33
  •  Unigene: 249184