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Home >> Antibodies >> MBD1 (Methyl-CpG Binding Domain Protein 1)

MBD1 (Methyl-CpG Binding Domain Protein 1)

Mouse Monoclonal Antibody [Clone CPTC-MBD1-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human colon stained with Methyl-CpG Binding Domain Protein 1 Mouse Monoclonal Antibody (CPTC-MBD1-1)

Product Details

Synonyms

CXXC-type zinc finger protein 3; CXXC3; MBD1; MECP1 COMPLEX; Methyl-CpG-binding domain protein 1 (MBD1); Protein containing methyl-CpG-binding domain 1 (PCM1); Regulator of fibroblast growth factor 2 (FGF 2) transcription (RFT)

Positive Control

Human tonsil or colon (IHC).

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MBD1 protein

Cellular Localization

Nuclear speckles. Chromosome.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

80kDa

Specificity & Comments

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has also been predicted to be involved in the development and progression of tumors, and it has been shown that MBD1 is significantly upregulated in pancreatic cancer tissues when compared with surrounding normal tissues. Furthermore, MBD1 may serve as a potential therapeutic target for pancreatic cancer.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hendrich, B. and Bird, A. 1998. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol. Cell. Biol. 18: 6538-6547.
  2. Ng, H.H., et al. 1999. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nat. Genet. 23: 58-61.

Bioinformatics

  •  Entrez Gene ID: 4152
  •  Gene Symbol: MBD1
  •  SwissProt: Q9UIS9
  •  Chromosome Location: 18q21
  •  Unigene: 405610
NBT02271