Formalin-fixed, paraffin-embedded human adrenalgland stained with Interleukin-5 (IL-5) Mouse Monoclonal Antibody (IL5/4161). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified Interleukin-5 (IL-5) Mouse Monoclonal Antibody (IL5/4161). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Interleukin-5 (IL-5) Monospecific Mouse Monoclonal Antibody (IL5/4161).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. Native IL-5 is a disulfide-linked homodimer. IL-5 is initially synthesized as a precursor with a 19 amino acid signal peptide which is cleaved to form a 112 amino acid mature protein. Murine and human IL-5 exhibit 70% sequence identity at the amino acid level. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an chain that binds IL-5 with low affinity and a chain that does not bind IL-5, but together with the IL-5 a chain, constitutes the high affinity IL-5 receptor. The chain is common to the IL-3, IL-5 and GM-CSF receptors and has been shown to signal through the JAK/Stat pathway.