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Home >> Antibodies >> Interleukin-5 (IL-5)

Interleukin-5 (IL-5)

Mouse Monoclonal Antibody [Clone IL5/4161]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human adrenalgland stained with Interleukin-5 (IL-5) Mouse Monoclonal Antibody (IL5/4161). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified Interleukin-5 (IL-5) Mouse Monoclonal Antibody (IL5/4161). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Interleukin-5 (IL-5) Monospecific Mouse Monoclonal Antibody (IL5/4161).

Product Details

Synonyms

B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; Interleukin 5 (colony stimulating factor, eosinophil); T Cell Replacing Factor; TRF

Positive Control

Human thymus or tonsil tissue.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human IL5 protein corresponding to the mature protein (exact sequence is proprietary)

Cellular Localization

Secreted. Extracellular space.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. Native IL-5 is a disulfide-linked homodimer. IL-5 is initially synthesized as a precursor with a 19 amino acid signal peptide which is cleaved to form a 112 amino acid mature protein. Murine and human IL-5 exhibit 70% sequence identity at the amino acid level. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an chain that binds IL-5 with low affinity and a chain that does not bind IL-5, but together with the IL-5 a chain, constitutes the high affinity IL-5 receptor. The chain is common to the IL-3, IL-5 and GM-CSF receptors and has been shown to signal through the JAK/Stat pathway.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Campbell H.D., et al.Proc Natl Acad Sci U S A. 1987 Oct;84(19):6629-33. doi: 10.1073/pnas.84.19.6629.

Bioinformatics

  •  Entrez Gene ID: 3567
  •  Gene Symbol: IL5
  •  SwissProt: IDP05113
  •  Chromosome Location: 5q31.1
  •  Unigene: 2247