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Interleukin-5 (IL-5) Antibody [IL5/4161]

In Stock
Catalog Number Formulation Size Price
3567-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3567-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3567-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. Native IL-5 is a disulfide-linked homodimer. IL-5 is initially synthesized as a precursor with a 19 amino acid signal peptide which is cleaved to form a 112 amino acid mature protein. Murine and human IL-5 exhibit 70% sequence identity at the amino acid level. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an chain that binds IL-5 with low affinity and a chain that does not bind IL-5, but together with the IL-5 a chain, constitutes the high affinity IL-5 receptor. The chain is common to the IL-3, IL-5 and GM-CSF receptors and has been shown to signal through the JAK/Stat pathway.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Secreted
Gene Name
IL5
Positive Control
Human thymus or tonsil tissue.
Immunogen
Recombinant fragment of human IL5 protein corresponding to the mature protein (exact sequence is proprietary)
Alternate Names
Interleukin-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; Interleukin 5 (colony stimulating factor, eosinophil); T Cell Replacing Factor; TRF

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
IL5/4161
Chromosome Location
5q31.1
Mol. Weight of Antigen
17kDa

Functions

  • Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Key References

  • Campbell H.D., et al.Proc Natl Acad Sci U S A. 1987 Oct;84(19):6629-33. doi: 10.1073/pnas.84.19.6629.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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