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HNF1A (Pancreatic Tumor Suppressor) Antibody [HNF1A/2087]

In Stock
Catalog Number Formulation Size Price
6927-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6927-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6927-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

HNF1A belongs to the homeobox protein family and is an essential transcription factor for many hepatic genes involved in detoxification, homeostasis and metabolisms of glucose, lipid, steroid and amino acid. In addition, HNF1A is an important component of the transcriptional networks governing embryonic pancreas development and differentiation. as well as maintaining the growth and function of islet cells in adult. HNF1A (Hepatocyte nuclear factor 1 alpha) is a transcription factor that is known to regulate pancreatic differentiation and maintain homeostasis of endocrine pancreas. Recently, genome-wide association studies have implicatedHNF1Aas a susceptibility gene for pancreatic cancer.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Jurkat cells. Small Intestine or Pancreas.
Immunogen
Recombinant fragment (around aa 214-339) of human HNF1A protein (exact sequence is proprietary)
Alternate Names
Hepatocyte nuclear factor 1-alpha, Liver-specific transcription factor LF-B1, Transcription factor 1, Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic transcription factor 1 alpha; HNF-1A; Interferon production regulator factor; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LFB1; Maturity onset diabetes of the young 3 (MODY3); TCF1; Transcription factor 1 hepatic

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
HNF1A/2087
Chromosome Location
12q24.31
Mol. Weight of Antigen
79kDa

Functions

  • Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:12453420, PubMed:10966642). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Key References

  • Bach, I., et al. 1993. EMBO J. 12: 4229-4242.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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