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Home >> Antibodies >> Filaggrin (Keratinocyte Differentiation Marker)

Filaggrin (Keratinocyte Differentiation Marker)

Recombinant Mouse Monoclonal Antibody [Clone rFLG/1561]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561).

img

SDS-PAGE Analysis Purified Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin

Positive Control

U-251-MG cells. Skin.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26-45kDa (Processed); 350kDa (Profilaggrin)

Specificity & Comments

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gan, S.Q., et al. 1990. Organization, structure, and polymorphisms of thehuman profilaggrin gene. Biochemistry 29: 9432-9440.

Bioinformatics

  •  Entrez Gene ID: 2312
  •  Gene Symbol: FLG
  •  SwissProt: P20930
  •  Chromosome Location: 1q21.3
  •  Unigene: 654510
NBT01946