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Home >> Antibodies >> CD74 (B-Cell Marker) (Transcription Regulator)

CD74 (B-Cell Marker) (Transcription Regulator)

Mouse Monoclonal Antibody [Clone CLIP/6609]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human lymph node stained with CD74 Mouse Monoclonal Antibody (CLIP/6609) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human tonsil stained with CD74 Mouse Monoclonal Antibody (CLIP/6609) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

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SDS-PAGE Analysis Purified CD74 Mouse Monoclonal Antibody (CLIP/6609). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Positive Control

293T, Daudi or Raji cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD74 protein (exact sequence is proprietary)

Cellular Localization

Cell surface. Paranuclear globular.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

33-41kDa

Specificity & Comments

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
  2. Marder RJ et. al. Lab Invest 52: 497-504, 1985.

Bioinformatics

  •  Entrez Gene ID: 972
  •  Gene Symbol: CD74
  •  SwissProt: P04233
  •  Chromosome Location: 5q33.1
  •  Unigene: 436568