Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Mouse Monoclonal Antibody (C68/684).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (C68/684).
SDS-PAGE Analysis Purified CD68 Mouse Monoclonal Antibody (C68/684).
Western Blot Analysis of human Spleen tissue lysate using CD68 Mouse Monoclonal Antibody (C68/684).
Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot
Flow Cytometric Analysis of U87MG cells using CD163 Mouse Monoclonal Antibody (M130/3296) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocyticneoplasia. Lymphomas are negative or show few granules.