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Home >> Antibodies >> CD59 / Complement Regulatory Protein / Protectin

CD59 / Complement Regulatory Protein / Protectin

Mouse Monoclonal Antibody [Clone SPM616]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Monoclonal Antibody (SPM616)

Product Details

Synonyms

20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein

Positive Control

Jurkat or Raji cells. Human lymphocytes. Human lymph node and tonsil.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF, IHC-P         More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD59 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. A E Fritzinger, F. Marciano-Cabral, et al. (2006) Infection and Immunity 74(2):1189-1195.
  2. J Zhang, C Gerhardinger, M Lorenzi (2002) Diabetes 51(12): 3499-3504

Bioinformatics

  •  Entrez Gene ID: 966
  •  Gene Symbol: CD59
  •  SwissProt: P13987
  •  Chromosome Location: 11p13
  •  Unigene: 278573, 709466
NBT00629