2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD53 (TSPAN25)

CD53 (TSPAN25)

Mouse Monoclonal Antibody [Clone 63-5A3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

SDS-PAGE Analysis Purified CD53 Mouse Monoclonal Antibody (63-5A3). Confirmation of Purity and Integrity of Antibody

Product Details

Synonyms

CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25

Positive Control

Daudi, Raji and Jurkat cells. Human leukocytes. Human tonsil and lymph node.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human Sezary cells

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

33-55kDa

Specificity & Comments

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995.
  2. Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997.
  3. Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989
  4. Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716.

Bioinformatics

  •  Entrez Gene ID: 963
  •  Gene Symbol: CD53
  •  SwissProt: P19397
  •  Chromosome Location: 1p13
  •  Unigene: 443057
NBT00356