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Home >> Antibodies >> CD48 (Pan Leukocyte Marker)

CD48 (Pan Leukocyte Marker)

Mouse Monoclonal Antibody [Clone CD48/4783]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human lymph node stained with CD48-Monospecific Mouse Monoclonal Antibody (CD48/4783) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human tonsil stained with CD48-Monospecific Mouse Monoclonal Antibody (CD48/4783) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Western blot analysis of Raji cell lysate using CD48 Mouse Monoclonal Antibody (CD48/4783).

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SDS-PAGE Analysis Purified CD48 Mouse Monoclonal Antibody (CD48/4783). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to CD48 (CD48/4783).

Product Details

Synonyms

B-cell activation marker; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; BLAST1; CD48 antigen (B cell membrane protein); Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2

Positive Control

Raji cells. Human lymph node or tonsil.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 59-178) of human CD48 protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, New York and London, 1997.
  2. Yokoyama S et al. Expression of the Blast-1 activation/adhesion molecule and its identification as CD48. J Immunol 1991, 146(7):2192-2200.

Bioinformatics

  •  Entrez Gene ID: 962
  •  Gene Symbol: CD48
  •  SwissProt: P09326
  •  Chromosome Location: 1q21.3-q22
  •  Unigene: 243564