Recombinant CD48 (Pan Leukocyte Marker) [CD48/8360R]

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Summary

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cell surface.
Gene Name
Positive Control
Raji cells. Human lymph node or tonsil.
Immunogen
Recombinant fragment (around aa 59-178) of human CD48 protein (exact sequence is proprietary)
Alternate Names
CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, B-cell activation marker; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; BLAST1; CD48 antigen (B cell membrane protein); Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2

Database Links

Entrez Gene ID
962
Unigene
SwissProt

Additional Information

Clone
CD48/8360R
Human Gene Symbol
CD48
Chromosome Location
1q21.3-q22
Mol. Weight of Antigen
45kDa

Functions

  • Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation (PubMed:27249817, PubMed:12007789). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:9841922, PubMed:27249817).

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, New York and London, 1997.
  • Yokoyama S et al. Expression of the Blast-1 activation/adhesion molecule and its identification as CD48. J Immunol 1991, 146(7):2192-2200.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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