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Recombinant CD48 (Pan Leukocyte Marker) Antibody [CD48/8360R]

Additional information

Catalog Number: 962-RBM10
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
962-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
962-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
962-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD48 (Pan Leukocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD48 Recombinant Rabbit Monoclonal Antibody (CD48/8360R). Inset: PBS instead of primary antibody; secondary only negative control.

CD48 Antibody in Western Blot (WB)

CD48 Antibody in Western Blot (WB)

Western blot analysis of Raji cell lysate using CD48 Recombinant Rabbit Monoclonal Antibody (CD48/8360R).

CD48 Antibody in SDS-PAGE

CD48 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD48 Recombinant Rabbit Monoclonal Antibody (CD48/8360R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CD48/8360R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell surface.
Gene Name
CD48
Positive Control
Raji cells. Human lymph node or tonsil.
Immunogen
Recombinant fragment (around aa 59-178) of human CD48 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation (PubMed:27249817, PubMed:12007789). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:9841922, PubMed:27249817).
Key References
  • Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, New York and London, 1997.
  • Yokoyama S et al. Expression of the Blast-1 activation/adhesion molecule and its identification as CD48. J Immunol 1991, 146(7):2192-2200.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
962
SwissProt
P09326
Chromosome Location
1q21.3-q22
Mol. Weight of Antigen
45kDa
Alternate Names
CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, B-cell activation marker; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; BLAST1; CD48 antigen (B cell membrane protein); Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2

Summary

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

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