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Home >> Antibodies >> CD40 / TNFRSF5 / CD40L-Receptor

CD40 / TNFRSF5 / CD40L-Receptor

Mouse Monoclonal Antibody [Clone C40/2383]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human tonsil stained with CD40 Mouse Monoclonal Antibody (C40/2383).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD40 Mouse Monoclonal Antibody (C40/2383).

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Flow Cytometric Analysis of U2OS cells using Monospecific Mouse Monoclonal Antibody to CD40 (C40/2383) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified CD40 Mouse Monoclonal Antibody (C40/2383). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to CD40 (C40/2383).

Product Details

Synonyms

B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; GP39; HIGM1; IGM; IMD3; p50; TBAM; TNF receptor superfamily member 5; TNFRSF5; TRAP

Positive Control

U2OS or L-428 cells. Human tonsil, spleen or thymus.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

Cellular Localization

Cell Surface. Secreted.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

43kDa

Specificity & Comments

CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kehry, M.R., et al. 1994. B cell activation by helper T cell membranes. Crit. Rev. Immunol. 14: 221-238.

Bioinformatics

  •  Entrez Gene ID: 958
  •  Gene Symbol: CD40
  •  SwissProt: P25942
  •  Chromosome Location: 20q12-q13.2
  •  Unigene: 472860