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CD40, Mouse Antibody [FGK45.5]

Additional information

Catalog Number: RTM1-2005
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
RTM1-2005-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
RTM1-2005-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
RTM1-2005-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD40 Antibody in SDS-PAGE

CD40 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD40 Rat Monoclonal Antibody (FGK45.5). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • Functional Studies
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Mouse
Clone
FGK45.5
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD40
Positive Control
MPBMC's. Spleen, Thymus or Lymph Node.
Immunogen
Purified recombinant Mouse CD40 fusion protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Key References
  • Agonistic Anti-CD40 Antibody Profoundly Suppresses the Immune Response to Infection with Lymphocytic Choriomeningitis Virus C. Bartholdy, et al. J. Immunol. 178 1662 (2007)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
21939
SwissProt
P27512
Chromosome Location
2 H3; 2 85.38 cM (Mouse)
Mol. Weight of Antigen
48kDa
Alternate Names
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, B cell surface antigen CD40, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TRAP, IMD3, IGM, Tnfrsf5, HIGM1, GP39, T-BAM

Summary

Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. FGK4.5 can be used for blocking of CD40/CD154 interaction and forin vitroandin vivoactivation of CD40 expressing APCs. This MAb is shown to indirectly activate natural killer (NK) cells, producing significant antitumor and anti-metastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.

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