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Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Mouse Monoclonal Antibody [Clone OKT3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified CD3e Mouse Monoclonal Antibody (OKT3). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD3 epsilon, CD3 TCR complex, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Positive Control

Jurkat cells. Tonsil and lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-Fr, IF, Func.         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (acetone fixed frozen sections)
Immunofluorescence (1-2ug/ml)
Functional Studies (Order Ab without Azide)
Optimal dilution for a specific application should be determined.

Immunogen

Human PBMCs

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

23kDa

Specificity & Comments

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

Related Products



200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Bagnara D, et al. 2011. Blood. 117: 5463-5472. (in vivo depletion)
  2. Haga-Friedman A, et al. Jun 2012. J. Immunol. 188:5538-5546. (in vitro activation)
  3. Roura-Mir C, et al. 2005. J. Immunol. 174:3773-3780 (IHC - Acetone fixed frozen sections)

Bioinformatics

  •  Entrez Gene ID: 916
  •  Gene Symbol: CD3E
  •  SwissProt: P07766
  •  Chromosome Location: 11q23.3
  •  Unigene: 3003
NBT00268