Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone C3e/4652R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human lymph node stained with CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong cell surface staining using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Western blot analysis of Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

Formalin-fixed, paraffin-embedded human lymph node stained with CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong cell surface staining using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Western blot analysis of Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

Product Details

Synonyms

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

Positive Control

Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human. Does not react with Cat or Dog.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

20kDa

Specificity & Comments

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.