2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD21 (Mature B-Cell & Follicular Dendritic Cell Marker)

CD21 (Mature B-Cell & Follicular Dendritic Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone CR2/3124R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).

img

SDS-PAGE Analysis Purified CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R). Confirmation of Purity and Integrity of Antibody.

img

Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

img

Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Product Details

Synonyms

CD21; Complement C3d receptor (C3DR); Complement Receptor type 2 (CR2); CVID7; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR; SLEB9

Positive Control

MOLT4, WEHI-231 cells and Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

140kDa

Specificity & Comments

Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Otsuka, M., et al. 2004. Br. J. Haematol. 127: 416-424.

Bioinformatics

  •  Entrez Gene ID: 1380
  •  Gene Symbol: CR2
  •  SwissProt: P20023
  •  Chromosome Location: 1q32
  •  Unigene: 445757
NBT03124