Formalin-fixed, paraffin-embedded human spleen stained with CD21 Recombinant Mouse Monoclonal Antibody (rCR2/1952).

SDS-PAGE Analysis Purified CD21 Recombinant Mouse Monoclonal Antibody (rCR2/1952). Confirmation of Integrity and Purity of Antibody.

Printable Datasheet

Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) [rCR2/1952]

Name: CD21 (Mature B-Cell &amp; Follicular Dendritic Cell Marker)
SKU: 1380-MSM11
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

Recognizes a protein of 140kDa, which is identified as the complement receptor 2(CR2)/CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro.CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Product Properties & Targets

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Cell membrane

Light Chain

Kappa

Gene Name

CR2

Positive Control

WEHI-231 cells. Human tonsil (IHC).

Immunogen

Recombinant fragment (around aa 44-196) of human CR2 (CD21) protein (exact sequence is proprietary)

Alternate Names

Complement receptor type 2, Complement C3d receptor, Epstein-Barr virus receptor, CD21; Complement C3d receptor (C3DR); Complement Receptor type 2 (CR2); CVID7; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR; SLEB9

Database Links

Entrez Gene ID

1380

Unigene

445757

SwissProt

P20023

Additional Information

Clone

rCR2/1952

Human Gene Symbol

CR2

Chromosome Location

1q32

Mol. Weight of Antigen

140kDa

Functions

Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).
(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Otsuka, M., et al. 2004. Role of CD21 antigen in diffuse large B-cell lymphoma and its clinical significance. Br. J. Haematol. 127: 416-4

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA &azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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