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Home >> Antibodies >> Catenin, beta (p120)

Catenin, beta (p120)

Recombinant Rabbit Monoclonal Antibody [Clone CTNNB1/2030R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-Catenin Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).

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Western Blot Analysis of HeLa cell lysate using Beta-Catenin Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).

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SDS-PAGE Analysis Purified Beta-Catenin Recombinant Rabbit Monoclonal Ab (CTNNB1/2030R). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of PFA fixed HeLa cells using Beta-Catenin Recombinant Rabbit Monoclonal Ab (CTNNB1/2030R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence Analysis of PFA fixed HeLa cells labeling Beta-Catenin with Beta-Catenin Recombinant Rabbit Monoclonal Ab (CTNNB1/2030R followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Catenin, beta (CTNNB1) Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).

Product Details

Synonyms

Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Positive Control

HeLa or MCF-7 cells. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human β-catenin protein

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

92kDa

Specificity & Comments

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

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200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437.
  2. Sarrio D et. al. Oncogene. 2004;23:3272-3283.

Bioinformatics

  •  Entrez Gene ID: 1499
  •  Gene Symbol: CTNNB1
  •  SwissProt: P35222
  •  Chromosome Location: 3p22.1
  •  Unigene: 476018
NBT02030